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Changes in heart rate (fH) and related parameters when Atlantic salmon were given a critical swim (Ucrit) test (water velocity increment of 0.2 BL s−1). a fH (in bpm) was measured every 2 min in salmon immediately prior to (resting values) and during the Ucrit test. Heart rate variability (HRV, in ms); b was manually calculated from the electrocardiograms and the percentage of ‘good’ quality ECGs (i.e., QI0); c was calculated by the tag algorithm. Dissimilar lower case letters indicate a significant difference between values, as determined by linear mixed-effects models. These models did not include the swimming speeds of 1.8 and 2.0 BL s−1 due to low sample sizes. The percentage of good quality ECGs did not change significantly with swim speed (P > 0.05). Data are means ± S.E.M.; n = 6–8 except where indicated
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Several companies are producing biologging equipment/sensors that require validation . The new centi-HRT ACT tag developed by Star-Oddi (Iceland) can simultaneously measure fH, ECGs, tri-axial acceleration and temperature (see for specifics on recording capabilities). There are few tags available that simultaneously measure both physiological and behavioral parameters, and only one study exists in which a DST recorded both fH and acceleration parameters in salmonids. Clark et al. defined relationships between acceleration, fH, tail beat frequency, energy expenditure and swimming speed in free-swimming sockeye salmon. However, the data logger used in their study (iLogR, B.D. Taylor, La Trobe University, Melbourne, Australia) is not commercially available. While several studies have used Star-Oddi milli-HRT tags to measure fH in fishes , it is not currently known how accurate or effective the centi-HRT ACT tags are for monitoring the fH and activity of free-swimming fish.
The implantation of tags requires that procedures such as netting, handling and anesthesia be used, and that the fish undergo surgery, which are all known to be stressful on the fish being tagged . Conveniently, the parameters being recorded by the centi-HRT ACT tag (i.e., fH and activity) provide information about the impact of surgery and can help to establish recommendations for recovery time post-implantation. For instance, it is well known that fH indicates the level of stress experienced by fish, and thus, it can be used to determine/estimate recovery time following a variety of stressors (e.g., see ). In Experiment #2, it appeared that the salmon’s fH began to stabilize within the first 4 days following surgical implantation (Fig. 5). This timeframe is similar to that reported by Brijs et al. for the fH of rainbow trout tagged with milli-HRT tags. However, when holding salmon for extended periods during Experiment #3, it was revealed that the fH of the salmon continued to decline for up to 2–3 weeks (Fig. 7). The reason(s) for this prolonged recovery period is/are not known. However, these data are consistent with Hvas et al. , and recent data suggest that the presence of tags inside the body cavity induces a long-term immune response . This latter finding may provide an explanation for the lengthy period required for fH to achieve stable values.
Brijs J, Sandblom E, Axelsson M, Sundell K, Sundh H, Huyben D, Brostrӧm R, Kiessling A, Berg C, Grӓns A. The final countdown: continuous physiological welfare evaluation of farmed fish during common aquaculture practices before and during harvest. Aquaculture. 2018;495(1):903–11.
